Pro-inflammatory cytokines are the cytokines that promotes systemic inflammation. Inflammation is a beneficial host response to a foreign challenge or tissue injury that ultimately leads to the restoration of normal tissue structure and function. Prolonged inflammation contributes to the pathogenesis of many inflammatory diseases. The main pro-inflammatory mediators include cytokines, primarily TNF- α, IL-1, and IL-6. We have studied the effect of GutGard® on the release of IL-1 β, IL-6 and NO in LPS-induced murine macrophages.